McCann, A., Ø. Midttun, K.C. 2020 Dec 22. doi: 10.1111/nyas.14547. G. S. HO, Y. H. CHONG, B.SC., PH.D., M.I. The measurement of erythrocyte transketolase activity enables evaluation of the thiamine status and therapeutic decisions in the disorders of the nervous system related to its deficiency. A method for measuring erythrocyte transketolase activity (ETKA) is described that is precise, economical of reagents and capable of high throughput with partial or full automation. Hiffler, L., B. Rakotoambinina, N. Lafferty, et al. Erythrocyte transketolase levels reliably detect thiamine deficiency but are not necessary for the diagnosis of WE. From clinical evidence these subjects were divided into five groups: (1) necropsy-proved cases of subacute … Symptoms of classic thiamine deficiency are recognized as beriberi, although clinical symptoms are nonspecific and recognition of subclinical deficiency is difficult. Keywords: COVID-19 is an emerging, rapidly evolving situation. Herve C, Beyne P, Lettéron P, Delacoux E. Clin Chim Acta. It mediates transfer of CH2OHC=O between donor and acceptor sugars. Erythrocyte transketolase activity, markers of cardiac dysfunction and the diagnosis of infantile beriberi. ETKAC; beriberi; thiamine; vitamin B1.  |  To purchase short term access, please sign in to your Oxford Academic account above. Erythrocyte transketolase (TK; EC 2.2.1.1), a thiamine-dependent enzyme, is widely used clinically for assessing thiamine status, defi­ ciency ofwhich causes a decrease in the enzyme activity. Please check your email address / username and password and try again. 1. NLM Thiamine deficiency disorders: diagnosis, prevalence, and a roadmap for global control programs. Copyright © 1970 by The American Society for Clinical Nutrition, Inc. You do not currently have access to this article. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. BIOL., F.R.I.C., Erythrocyte Transketolase Activity, The American Journal of Clinical Nutrition, Volume 23, Issue 3, March 1970, Pages 261–266, https://doi.org/10.1093/ajcn/23.3.261. The measurement of erythrocyte transketolase activity enables evaluation of the thiamine status and therapeutic decisions in the disorders of the nervous system related to its deficiency. The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for deficiency, partly due to a lack of assay harmonization. Select a category... Transketolase Thiamine Pyrophosphate Thiamine Deficiency Transaldolase Thiamine Oxythiamine Pentoses Pentosephosphates Thiamine Pyrophosphate Thiamine Deficiency Transaldolase Thiamine Oxythiamine Pentoses Pentosephosphates La transchetolasi è un enzima appartenente alla classe delle transferasi, che catalizza le seguenti reazioni: . Please enable it to take advantage of the complete set of features! Sci. Erythrocyte transketolase activity and guanidino compounds in hemodialysis patientsBackground The toxic effects of guanidino compounds on enzymatic activity in uremic patients are known. arczewski, A. Dobrowolska, et al. Erythrocyte transketolase activity and the effect of adding thiamine pyrophosphate (% thiamine pyrophosphate effect) were measured in 111 subjects suspected to suffer from Leigh's disease (subacute necrotising encephalomyelopathy). 2MRC Elsie Widdowson Laboratory, Cambridge, UK The erythrocyte transketolase activity and the effect of thiamin pyrophosphate stimulation (TPP effect) on this enzyme have been determined for about 360 Malaysians who included members of the military and police forces, athletes, laboratory personnel, prison inmates, pregnant women, and several beriberi patients. 2020 Dec;103(6):2600-2604. doi: 10.4269/ajtmh.20-0479. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Erythrocyte transketolase activity (ETKA) has been studied in a group of uremic patients exhibiting neuropathy on clinical grounds and demonstrating … Treasure Island (FL): StatPearls Publishing; 2020 Jan–. Erythrocyte transketolase activity and its stimulation in vitro by the addition of thiamine pyrophosphate (TPP effect) was measured in 64 normally nourished alcoholics with well-compensated liver disease and in 20 control subjects. xilulosio 5-fosfato + ribosio 5-fosfato ⇄ gliceraldeide 3-fosfato + sedoeptuloso 7-fosfato. This enzyme is important in erythrocyte metabolism and its activity can therefore be used as an index of thiamine nutrition. Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status Kerry S.Jones, 1Damon A.Parkington, Lorna J.Cox,1,2 and Albert Koulman1 1NIHR BRC Nutritional Biomarker Laboratory, MRC Epidemiology Unit, University of Cambridge, Cambridge, UK. Trasferisce un frammento a due atomi di carbonio dallo xilulosio-5-fosfato al ribosio-5-fosfato. British Journal of Nutrition (1994), 72, 11 1-125 111 Thiamin intake, erythrocyte transketolase (EC 2.2.1.1) activity and total erythrocyte thiamin in adolescents BY ANGELA L. BAILEY, P. M. FINGLAS, A. J. Basal and activated erythrocyte transketolase activities (ETK) and activation (α) coefficients were assayed along with plasma brain natriuretic peptide, N-terminal pro-brain natriuretic peptide and troponin T. Basal ETK (and to a lesser extent activated ETK) and plasma troponin T were the only infant biochemical markers that predicted infantile beriberi. The ETK activity assay as an indirect, functional marker of thiamine status has been used for over 50 years. 2017. Basal and activated erythrocyte transketolase activities (ETK) and activation (α) coefficients were assayed along with plasma brain natriuretic peptide, N-terminal pro-brain natriuretic peptide and troponin T. Basal ETK (and to a lesser extent activated ETK) and plasma troponin T were the only infant biochemical markers that predicted infantile beriberi. Thiamin in clinical practice. Thiamine deficiency in tropical pediatrics: new insights into a neglected but vital metabolic challenge. Transketolase activity is decreased in deficiency of thiamine, which in general is due to malnutrition. Erythrocyte transketolase activity in patients with diabetic and alcoholic neuropathies. Frank, L.L. activity transketolase. The erythrocyte transketolase activity and the effect of thiamin pyrophosphate stimulation (TPP effect) on this enzyme have been determined for about 360 Malaysians who included members of the military and police forces, athletes, laboratory personnel, prison inmates, pregnant women, and several beriberi patients. Clipboard, Search History, and several other advanced features are temporarily unavailable. Nixon PF, Price J, Norman-Hicks M, Williams GM, Kerr RA. The activity of erythrocyte’s transketolase in IBD patients (n=49). Thiamine measurement in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia Online ahead of print. 39: 503-520. BIOL., F.R.I.C. The aim of the study was to compare different modes of expression of transketolase activity in the most frequent acquired neuropathies. Mentioning: 11 - Erythrocyte transketolase activity and its stimulation in vitro by the addition of thiamine pyrophosphate (TPP effect) was measured in 64 normally nourished alcoholics with well-compensated liver disease and in 20 control subjects. Erythrocyte transketolase deficiency in patients suffering from Crohn’s disease. Biochemical evidence of thiamine deficiency as judged by low transketolase activity was found in 19 alcoholics (29.7%). The relationships between thiamin intake, erythrocyte transketolase (EC 2.2.1.1) activity coefficient (ETK-AC) and total erythrocyte thiamin were investigated in a group of adolescents (13 to 14 years old; nineteen boys, thirty-five girls). This article is also available for rental through DeepDyve. (trans″kēt'ol-ās″) The degree to which transketolase, an enzyme involved in carbohydrate metabolism, rises in response to a loading dose of thiamine pyrophosphate (TPP). 1990 Nov 30;192(2):89-98. doi: 10.1016/0009-8981(90)90072-z. Erythrocyte Transketolase Activity, Markers of Cardiac Dysfunction and the Diagnosis of Infantile Beriberi By Douangdao Soukaloun, Sue J. Lee, Karen Chamberlain, Ann M. Taylor, Mayfong Mayxay, Kongkham Sisouk, Bandit Soumphonphakdy, Khaysy Latsavong, Kongsin Akkhavong, Douangkham Phommachanh, Vanmaly Sengmeuang, Khonsavanh Luangxay, … 2nd ed. Relationship between calculated and analysed thiamin intakes for fifty-four adolescents aged 13-14 years. Here, we provide a step-by-step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient, including detailed explanations of equipment and chemicals required and guidance for quality control procedures. 3. Bourassa, B. Adamolekun, et al. Using the guideline that a TPP effect of greater than 25% is indicative of thiamin deficiency, we found that a significant proportion of our apparently healthy subjects (between 21 and 36%), may be regarded as thiamin deficient. Annals of the New York Academy of Sciences published by Wiley Periodicals, LLC on behalf of New York Academy of Sciences. Therefore, reliable biomarkers of thiamine status are required. Mestyán I, Jobst K, Hazafi K, Green A. Acta Medica Hungarica, 01 Jan 1986, 43(3): 315-319 PMID: 3588170 . D. a 8kowska-Wierzbicka, S. ichalak, . The transketolase-dependent transformation of ribose-5-phosphate,yielding glyceraldehyde-3-phosphate,is linked via 'indicator 2020 Jun 22. 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